anti gad1 antibody  (Bioss)

Journal: Heliyon

Article Title: A GAD1 inhibitor suppresses osteosarcoma growth through the Wnt/β-catenin signaling pathway

doi: 10.1016/j.heliyon.2024.e31444

Figure Lengend Snippet: GAD1 overexpression is related to a poor prognosis for OS patients. (A) Metastasis-free survival (MFS) results for 16 and 56 patients with elevated and decreased GAD1 expression, respectively; (B) Expression levels of GAD1 in normal bone and OS cell lines; (C) IHC staining showing GAD1 expression levels in human OS specimens and matched neighboring tissues. Scale bars, 100 μm; (D–E) Western blotting was performed to determine GAD1 levels in OS cell lines and hFoB1.19 cells. *** p < 0.001; NS, not significant.

Article Snippet: Immunohistochemistry (IHC) analysis with an anti-GAD1 antibody (Bioss, China, bs-1302R) was used to determine the GAD1 expression levels.

Techniques: Over Expression, Expressing, Immunohistochemistry, Western Blot

Journal: Science Advances

Article Title: A causal coding variant regulating alternative splicing of DOC2A at 16p.11.2 GWAS locus influences susceptibility to schizophrenia

doi: 10.1126/sciadv.adw7667

Figure Lengend Snippet: ( A ) Representative traces of mEPSC in primary hippocampal neuron infected with control (empty vector), DOC2A Full-Length , or DOC2A ∆Val217-Pro218 AAV. ( B and C ) Quantification of mEPSC amplitude and frequency. N ≥ 29 neurons. ( D ) Representative traces of mIPSC in in primary hippocampal neuron infected with control, DOC2A Full-Length , or DOC2A ∆Val217-Pro218 AAV. ( E and F ) Quantification of mIPSC amplitude and frequency. N ≥ 20 neurons. ( G ) Representative gels of Western blot. ( H to K ) Quantification of synaptophysin, VAMP-2, GAD65, and GAD67 expression in hippocampus tissue overexpressing control, DOC2A Full-Length , or DOC2A ∆Val217-Pro218 . N = 3 in each group. Full Western blotting images are shown in fig. S16. Data are presented as the means ± SEM; * P < 0.05, ** P < 0.01, and *** P < 0.001. Error bars indicate the SEM.

Article Snippet: The primary antibodies were as follows: FLAG (Cell Signaling Technology, no. 8146S; 1:1000) and DOC2A (Invitrogen, no. PA5-31345; 1:1000), GAD67 (Proteintech, no. 10408-1-AP; 1:5000), GAD65 (Cell Signaling Technology, no. 5843T; 1:1000), synaptophysin (Sigma-Aldrich, no. S5768; 1:500), VAMP-2 (Synaptic Systems, no. 104211; 1:2000), α-tubulin (Proteintech, no. 66031-1-Ig; 1:20,000), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase; Proteintech, no. 10494-1-AP; 1:20,000).

Techniques: Infection, Control, Plasmid Preparation, Western Blot, Expressing

Journal: bioRxiv

Article Title: A selective top-down pathway from anterior cingulate cortex embeds a representation of saliency in hippocampus

doi: 10.1101/2025.06.26.661852

Figure Lengend Snippet: (A) Dual retrograde labeling of ACC neurons. Left: The injection sites of retrograde AAVs in RSCd, RSCg and dmSTR. Right: The distribution of fluorescently tagged neurons in the ACC. (B) The proportion of dmSTR-projecting, RSCd-projecting, RSCg-projecting ACC neurons located in ACC layer 2/3 or layer 5. (C) The fraction of ACC projection neurons which project to each area. (D) Example images of sections of ACC labeled with retrograde AAV injected in RSCd/g and immunostained with anti-CaMKIIα or anti-GAD67 antibodies. (E) Similar to (D) but injections of retrograde AAV were targeted to dmSTR. (F) The proportion of RSCd/g-projecting or dmSTR-projecting ACC neurons which were labeled with anti-CaMKIIα or anti-GAD67.

Article Snippet: Prior to staining, slices were blocked in blocking buffer (0.1 M Tris-HCl, 0.15 M NaCl, 0.5% (w/v) blocking reagent (41116100, Roche)) at room temperature (RT) for 1 h. Slices were then washed with TNB-T (0.1 M Tris-HCl (pH 7.5), 0.15 M NaCl, 0.5% (w/v) blocking reagent, 1% Triton (R) X-100) twice for 2 min each and incubated with a monoclonal mouse anti-CaMKIIα antibody (1:250; 6G9, Abcam), or a polyclonal rabbit anti-GAD67 antibody (1:300; GTX 101881), or a monoclonal rabbit anti-c-Fos antibody (1:1,000; 9F6 #2250, Cell Signaling) in TNB-T overnight at 4 °C.

Techniques: Labeling, Injection